Publication:


abstract

The anterior subventricular zone of the adult mammalian brain contains progenitor cells which are upregulated after cerebral ischemia. We have previously reported that while a part of the progenitors residing in adult monkey anterior subventricular zone travels to the olfactory bulb, many of these cells sustain location in the anterior subventricular zone for months after injury, exhibiting a phenotype of either neural or neuronal precursors. Here we show that ischemia increased the numbers of anterior subventricular zone progenitor cells expressing developmentally regulated Transcription factors including Pax6 (paired-box 6), Emx2 (empty spiracles-homeobox 2), Sox 1-3 (sex determining region Y-box 1-3), Ngn1 (neurogenin 1), Dlx1,5 (distalless-homeobox 1,5), Olig1,3 (oligodendrocyte lineage gene 1,3) and Nkx2.2 (Nk-box 2.2), as compared with control brains. Analysis of transcription factor protein expression by sustained neural or neuronal precursors in anterior subventricular zone revealed that these two cell types were positive for characteristic sets of transcription factors. The proteins Pax6, Emx2, Sox2,3 and Olig1 were predominantly localized to dividing neural precursors while the factors Sox1, Ngn1, Dlx1,5, Olig2 and Nkx2.2 were mainly expressed by neuronal precursors. Further, differences between monkeys and non-primate mammals emerged, related to expression patterns of Pax6, Olig2 and Dlx2. Our results suggest that a complex network of developmental signals might be involved in the specification of primate progenitor cells.

Related Articles
Differential neurogenic potential of progenitor cells in dentate gyrus and CA1 sector of the postischemic adult monkey hippocampus.
Exp Neurol. 2006
Differential neurogenic potential of progenitor cells in dentate gyrus and CA1 sector of the postischemic adult monkey hippocampus.
Tonchev AB, Yamashima T. Exp Neurol. 2006 Mar; 198(1):101-13. Epub 2006 Jan 19.
Sox3 expression identifies neural progenitors in persistent neonatal and adult mouse forebrain germinative zones.
J Comp Neurol. 2006
Sox3 expression identifies neural progenitors in persistent neonatal and adult mouse forebrain germinative zones.
Wang TW, Stromberg GP, Whitney JT, Brower NW, Klymkowsky MW, Parent JM. J Comp Neurol. 2006 Jul 1; 497(1):88-100.
Enriched environment increases neural stem/progenitor cell proliferation and neurogenesis in the subventricular zone of stroke-lesioned adult rats.
Stroke. 2005
Enriched environment increases neural stem/progenitor cell proliferation and neurogenesis in the subventricular zone of stroke-lesioned adult rats.
Komitova M, Mattsson B, Johansson BB, Eriksson PS. Stroke. 2005 Jun; 36(6):1278-82. Epub 2005 May 5.
Review Gene profiles within the adult subventricular zone niche: proliferation, differentiation and migration of neural progenitor cells in the ischemic brain.
Curr Mol Med. 2007
Review Gene profiles within the adult subventricular zone niche: proliferation, differentiation and migration of neural progenitor cells in the ischemic brain.
Zhang RL, Zhang ZG, Chopp M. Curr Mol Med. 2007 Aug; 7(5):459-62.
Review Concise review: Pax6 transcription factor contributes to both embryonic and adult neurogenesis as a multifunctional regulator.
Stem Cells. 2008
Review Concise review: Pax6 transcription factor contributes to both embryonic and adult neurogenesis as a multifunctional regulator.
Osumi N, Shinohara H, Numayama-Tsuruta K, Maekawa M. Stem Cells. 2008 Jul; 26(7):1663-72. Epub 2008 May 8.
Anomaly in aortic arch alters pathological outcome of transient global ischemia in Rhesus macaques.
Brain Res. 2009
Anomaly in aortic arch alters pathological outcome of transient global ischemia in Rhesus macaques.
Hara K, Yasuhara T, Maki M, Matsukawa N, Yu G, Xu L, Tambrallo L, Rodriguez NA, Stern DM, Yamashima T, et al. Brain Res. 2009 Aug 25; 1286:185-91. Epub 2009 Jun 12.
Neurogenin2 directs granule neuroblast production and amplification while NeuroD1 specifies neuronal fate during hippocampal neurogenesis.
PLoS One. 2009
Neurogenin2 directs granule neuroblast production and amplification while NeuroD1 specifies neuronal fate during hippocampal neurogenesis.
Roybon L, Hjalt T, Stott S, Guillemot F, Li JY, Brundin P. PLoS One. 2009; 4(3):e4779. Epub 2009 Mar 10.
Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration.
Exp Eye Res. 2008
Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration.
Thummel R, Kassen SC, Enright JM, Nelson CM, Montgomery JE, Hyde DR. Exp Eye Res. 2008 Nov; 87(5):433-44. Epub 2008 Aug 5.