Publication:


abstract

tuberculosis is one of the oldest diseases known to affect humans and is caused by Mycobacterium tuberculosis. Lymph nodes are the most common site of extrapulmonary involvement. Tuberculous lymphadenitis can be presumptively diagnosed morphologically on fine-needle aspiration biopsy of lymph nodes. Additional tests like Ziehl-Neelsen (ZN) stain for detection of acid fast bacilli, fluorescence, and molecular markers are often employed for confirmation of diagnosis. However, culture is essential for obtaining a definitive diagnosis. Unfortunately, culture is time consuming and expensive. Newer investigative methods are required. The efficacy of Autofluorescence in the diagnosis of tuberculous lymphadenitis was evaluated for this purpose. Fine-needle aspiration biopsies were collected from patients with a clinical diagnosis of tuberculous lymphadenitis. Cytomorphological examination, ZN staining, autofluorescence, and culture were performed on all specimens. ZN staining was positive in 37.5% (30/80) of cases, while autofluorescence was positive in 57.5% (46/80) of patients and was found to have 81.8% specificity, 95% sensitivity, and a positive predictive value of 82.6%. This technique allowed rapid and early diagnosis of tuberculous lymphadenitis in a number of patients, permitting early institution of appropriate therapy. Diagn. Cytopathol. 2012; © 2012 Wiley Periodicals, Inc.

Copyright © 2012 Wiley Periodicals, Inc.

Related Articles
Diagnosing mycobacterial lymphadenitis in children using fine needle aspiration biopsy: cytomorphology, ZN staining and autofluorescence -- making more of less.
Diagn Cytopathol. 2008
Diagnosing mycobacterial lymphadenitis in children using fine needle aspiration biopsy: cytomorphology, ZN staining and autofluorescence -- making more of less.
Wright CA, van der Burg M, Geiger D, Noordzij JG, Burgess SM, Marais BJ. Diagn Cytopathol. 2008 Apr; 36(4):245-51.
Diagnosis of tuberculous lymphadenitis in an area of HIV infection and limited diagnostic facilities.
Trop Geogr Med. 1994
Diagnosis of tuberculous lymphadenitis in an area of HIV infection and limited diagnostic facilities.
Perenboom RM, Richter C, Swai AB, Kitinya J, Mtoni I, Chande H, Kazema RR, Mwakyusa DH, Maselle SY. Trop Geogr Med. 1994; 46(5):288-92.
Combining fine-needle aspiration biopsy (FNAB) and high-resolution melt analysis to reduce diagnostic delay in Mycobacterial lymphadenitis.
Diagn Cytopathol. 2010
Combining fine-needle aspiration biopsy (FNAB) and high-resolution melt analysis to reduce diagnostic delay in Mycobacterial lymphadenitis.
Wright CA, Hoek KG, Marais BJ, van Helden P, Warren RM. Diagn Cytopathol. 2010 Jul; 38(7):482-8.
Review [Diagnosis and treatment of tuberculous lymphadenitis of the neck].
Ned Tijdschr Geneeskd. 2000
Review [Diagnosis and treatment of tuberculous lymphadenitis of the neck].
van Loenhout-Rooyackers JH, Richter C. Ned Tijdschr Geneeskd. 2000 Nov 18; 144(47):2243-7.
Review Polymerase chain reaction vs. conventional diagnosis in fine needle aspirates of tuberculous lymph nodes.
Acta Cytol. 2001
Review Polymerase chain reaction vs. conventional diagnosis in fine needle aspirates of tuberculous lymph nodes.
Goel MM, Ranjan V, Dhole TN, Srivastava AN, Mehrotra A, Kushwaha MR, Jain A. Acta Cytol. 2001 May-Jun; 45(3):333-40.